Effects of Mtn Enzyme Deficiency on E. Coli O157:h7 Growth and Virulence
نویسندگان
چکیده
............................................................................................................................ vi LIST OF TABLES .................................................................................................................... x LIST OF FIGURES ................................................................................................................. xi LIST OF ABBREVIATIONS ................................................................................................ xiii CHAPTER ONE: INTRODUCTION ....................................................................................... 1 S-adenoyslmethionine Reactions .................................................................................. 2 Methylthioadenosine/S-adenosylhomocystiene Nucleosidase and the Methionine Salvage Pathway ........................................................................................................... 7 Quorum Sensing.......................................................................................................... 13 Biofilms....................................................................................................................... 17 Escherichia coli O157:H7........................................................................................... 19 Metabolics and Proteomics of E. coli RK4353 ........................................................... 25 Summary ..................................................................................................................... 28 CHAPTER TWO: MATERIALS AND METHODS ............................................................. 30 Creation of Electrocompetent Cells ............................................................................ 30 Generation of the MTN Knockout (MTN KO) Strain Using the λ-Red System ........ 30 Generation of the MTN Knock-In (MTN KI) Strain .................................................. 32 Analysis of MTN Activity: Preparation of Bacterial Lysates ..................................... 32
منابع مشابه
Prevalence of Virulence Genes of Escherichia Coli O157:H7 Isolated from Patients with Urinary Tract Infections in Shiraz, Iran
Abstract Background and Objective: Escherichia coli O157:H7 is one of the most well-known pathogenic bacteria worldwide that can develop severe diseases such as hemolytic uremic syndrome (HUS). This study aimed to assess the prevalence of virulence genes of E. coli O157:H7 in patients with suspected urinary tract infections (UTIs). Material and Methods: This cross-sectional study was co...
متن کاملStudy on the growth and survival of Escherichia coli O157:H7 during the manufacture and storage of Iranian white cheese in brine
The behaviour of Escherichia coli O157:H7 was studied during the manufacture and storage of Iranian white cheese in brine. Cheese was manufactured using pasteurized cow milk and inoculated with E. coli O157:H7 with inoculum level of 103 cfu/ml. Four treatments were designed. Cheeses were made with or without starter culture and kept immersed in 6 or 8% salt brine during ripening and storage. Ch...
متن کاملDefects in polynucleotide phosphorylase impairs virulence in Escherichia coli O157:H7
Polynucleotide phosphorylase (PNPase) is reported to regulate virulence in Salmonella, Yersinia sp. and Campylobacter jejuni, yet its role in Escherichia coli O157:H7 has not been investigated. To gain insights into its roles in E. coli O157:H7 virulence, pnp deletion mutants were generated and the major virulence factors were compared to their parental wild type strains. Deletion of pnp in E. ...
متن کاملModified Vero cell induced by Bifidobacterium bifidum inhibits enterohemorrhagic Escherichia coli O157:H7 cytopathic effect
Enterohemorrhagic Escherichia coli (EHEC), such as E. coli O157:H7, are emerging food-borne pathogens worldwide. This micro-organism can damage the epithelial tissue of the large intestine. The cytotoxic effects can be neutralized by probiotics such as Bifidobacterium bifidum. Probiotics are viable cells that have beneficial effects on the health of the host. The preventing activity of B. bifid...
متن کاملThe prevalence of Escherichia coli O157:H7, Listeria monocytogenes and Campylobacter spp. on bovine carcasses in Isfahan, Iran
This study was carried out to determine the prevalence of foodborne pathogens, Escherichia coli, E. coli O157:H7, Listeria monocytogenes and Campylobacter spp. on slaughtered cattle in Isfahan, Iran. A total of 203 cattle carcasses were sampled by surface section of neck meat taken immediately after slaughter and analyzed using microbiological examinations. Suspected colonies to E. coli O157:H7...
متن کامل